Resources

Baculovirus P10 Structures and Role in Insect Cells

A blog on baculovirus P10 structures and the role in insect cells might appear a slightly odd topic for a blog from a company based on expression of recombinant proteins. However, a recently published paper that is the product of a collaboration between the Insect Virus Research Group at Oxford Brookes University and OET Ltd offers some insight to the role of P10 structures in infected insect cells and why

baculoQUANT™ ALL-IN-ONE Titration of Recombinant Baculoviruses

There are many methods available for the titration of recombinant baculoviruses.  One of the most convenient is the use of the quantitative polymerase chain reaction (qPCR), which assesses how much virus DNA is present in a freshly-amplified stock.  This is compared with a standard curve derived from a control virus stock to give an infectious titre expressed as Q plaque forming units (pfu) per millilitre.  OET Ltd market the baculoQUANT™

Expression Systems Media

Expression Systems Insect Cell Culture Media OET is pleased to offer a wide range of Expression Systems Insect cell culture media, from the very popular ESF 921, to specialized insect transfection media. Expression Systems’ media are easy to adapt your cells into and the insect specific media gives great protein yield, when compared to rival media. ESF 921 Insect Culture Media OET use ESF 921 for all in-house work, due

Plaque-Assay Schematic

We’ve said it enough times; the plaque-assay is the ‘gold standard’ for determining virus infectivity. But this doesn’t make the procedure any easier! Whether it’s the agonising 5 day waiting period or the disorientating number of steps, plaque-assays can seem like a chore and we’ve heard of more than enough non-virologists admit to skipping this laborious technique in favour of a simpler solution. More often than not this isn’t a

How to Grow Cells over the Holiday Period

We want to talk about how to grow cells over the holiday period spanning Christmas and New Year.  (OK, so we have covered this before but we think it is worth repetition for new users of the baculovirus-insect cell system).  Most labs, academic and industry, will have some time off over the next few weeks. It is often a problem keeping your cell cultures in good condition so that when

Growing Mammalian Cells Successfully

While OET Ltd has built its reputation on baculovirus-insect cell expression systems, we also undertake work that requires growing mammalian cells successfully too.  Much of this work involves the transduction of these cells with BacMam vectors to express genes placed under the control of mammalian-specific promoter elements.  Some of our work in this area was recently published and involved the generation of improved BacMam vectors for transducing both mammalian cells in

Growing Insect Cells Successfully – Part II

In our previous blog we talked about the early stages of growing insect cells when you might just have revived a frozen stock and set up either monolayer or suspension cultures (or both).  If you have established a viable suspension culture – and here we are talking primarily about insect cells such as Sf21 or Sf9 – you will probably monitor them fairly closely for their rate of growth.  The

Growing Insect Cells Successfully

With Autumn, or Fall as some of you may call it, upon us everyone seems to back in action in the lab again and hopefully growing insect cells successfully.  Regular readers of these blogs will know that we frequently return to the question of how to propagate insect cells.  The reason for doing so is because growing insect cells successfully is fundamental to producing recombinant proteins using the baculovirus system.

Baculovirus Transfer Vector Plasmid Compatibility

A frequent customer query concerns baculovirus transfer vector plasmid compatibility with either flashBACTM or other systems for making recombinant viruses.  Obviously, if you are buying flashBACTM and our pOET range of transfer vectors you won’t have a problem.  However, given that baculovirus expression vectors have been around since 1983 and many labs have produced variations around a theme, it is not surprising that confusion can arise. Most baculovirus expression vectors

Pre-GMP Manufacture of Recombinant Proteins

While OET is currently unable to offer GMP manufacture of recombinant proteins or viruses, we can offer a premium service for pre-GMP manufacture.  This can serve as a very useful bridge to full GMP projects conducted by other service providers.  So what is the advantage of a pre-GMP step? It really boils down to cost.  Some of our own R&D is moving in the direction of needing GMP but we

Storing Cells at -80°C?

Every so often we are asked if storing insect cells at -80°C is a viable option if you haven’t got access to liquid nitrogen cryogenic facilities.  Our standard response is that this is not a good idea for long term storage of viable cells.  This is largely based on historical dogma that says that cells do not preserve their viability for long at these temperatures.  However, since one of our

When to Passage Sf9 Cells

An important factor in baculovirus expression is assessing when to passage Sf9 cells, which are commonly used to make recombinant viruses or for protein production.  In our last blog we talked about how to make the transition from monolayer to suspension cultures and how maintain cells in a healthy state during this time. To define what is meant by ‘healthy’ cells,  Figure 1 shows a sample of healthy Sf9 cells

Suspension Sf9 Cells from Monolayer Cultures

The transition to suspension Sf9 cells from monolayer cultures is not difficult but does depend on harvesting the attached cells at the right time. Too early and it is extremely hard to detach the cells from the culture flask. Too late and the cells will be in such poor condition that they may not recover when in suspension. Trying to describe in words how the monolayer culture should look prior

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