Titration of Virus Using baculoQUANT™ qPCR

OET has developed a rapid system based on quantitative PCR (qPCR) for the accurate titration of baculovirus stocks. In this method, the baculovirus DNA is purified from the budded virus using freshly amplified virus inoculum. The purified DNA is added to a qPCR reaction mix and then amplified in an ABI7500 Sequence Detection System. Following amplification, the quantity of virus particles is determined against the known standards that have been titrated by plaque assay. While this method has proven to be as accurate as plaque assay in our experience, it is not recommended for virus stocks older than three months. Spurious results are obtained from old virus stocks as a result of aggregation and degradation of the virus particles. A plaque assay can determine the titre of an old virus stock more accurately, click here to find out more out our plaque assay services.

Our service offers an optimised method, based on the plaque assay, to determine an accurate infectious titre of the recombinant baculovirus stock. Customers need to provide at least 0.5ml of virus inoculum for the baculoQUANT™ titration method.

The baculoQUANT™ titration kit is also available to buy from our online shop