Here at Oxford Expression Technologies, the mammalian expression projects are performed using plasmid constructs that can be used both for transient and stable expression in a number of different mammalian cell lines. Oxford Expression Technologies has access to a wide range of mammalian promoters to customise the level of gene expression in mammalian cells. Like all Oxford Expression Technologies contract services the baculovirus projects can cover every step from gene synthesis to protein optimisation, scale up and purification.
- Gene synthesis and gene cloning – We can arrange the synthesis of any gene for you, and we’ll also work with any plasmid you supply as a starting point.
- Transient expression – The plasmid containing the gene of interest is transfected into the target mammalian cell line and expression levels are determined by western blot.
- Stable cell line – Stable cell line is produced following transfection of mammalian cells with the plasmid containing the gene of interest and a selectable antibiotic marker. Positive selection is used by the presences of the antibiotic to isolate and amplify recombinant mammalian cells, which contain the gene of interest.
- Expression of recombinant protein – Following transient expression or generation of the stable cell line experiment, the protein produced can be purified or shipped direct as crude product.
Scale up – OET can scale up the production, all the way to 20L using a WAVE machine bioreactor.
These projects are designed to be highly flexible and each one can be tailored to your specific requirements.
Please contact us for further information or to request mammalian services quotation, email us on email@example.com now.
BacMam protein expression services
Oxford Expression Technologies also offers BacMam expression service
BacMam viruses can be constructed by OET using the flashBAC™ expression system. This service includes complete optimisation of expression for your protein of interest. BacMam protein expression services offer high quality proteins at a competitive price.
What are BacMam viruses?
BacMams are modified baculoviruses in which a mammalian promoter is used to drive gene expression in mammalian cells following baculoviral transduction. The system can be used to transduce a wide range of mammalian cell lines including HEK293, HeLa, U2-OS, COS and CHO cells. Transduction of mammalian cells with BacMam viruses has been shown to be highly efficient and dose-dependent. Moreover, the ability of baculoviruses to enter mammalian cells means that the transduction can be achieved by simply adding a virus inoculum to the cells.
What are the advantages of the BacMam expression system?
The BacMam system offers several advantages over plasmid based transfection and other virus based gene expression systems. The key advantages include:
Safe – high level of biosafety as baculoviruses do not replicate in mammalian cells
Low toxicity – reduced overt cytotoxicity even at high virus concentration
Efficient – high transduction efficiency maximises gene delivery into cells
Wide host range – a broad range of mammalian cell types can be transduced
Rapid – expression is much faster than generation of stable cell lines
Interested in BacMam expression? Contact us to find out how we can help.