BacMam expression service – baculovirus expression in mammalian cells
Interested in BacMam protein expression services? Contact us to find out how we can help.
BacMam viruses can be constructed by OET using the flashBAC™ expression system. This service includes complete optimisation of expression for your protein of interest. BacMam protein expression services offer high quality proteins at a competitive price.
What are BacMam viruses?
BacMams are modified baculoviruses in which a mammalian promoter is used to drive gene expression in mammalian cells following baculoviral transduction. The system can be used to transduce a wide range of mammalian cell lines including HEK293, HeLa, U2-OS, COS and CHO cells. Transduction of mammalian cells with BacMam viruses has been shown to be highly efficient and dose-dependent. Moreover, the ability of baculoviruses to enter mammalian cells means that the transduction can be achieved by simply adding a virus inoculum to the cells.
What are the advantages of the BacMam expression system?
The BacMam system offers several advantages over plasmid based transfection and other virus based gene expression systems. The key advantages include:
Safe – high level of biosafety as baculoviruses do not replicate in mammalian cells
Low toxicity – reduced overt cytotoxicity even at high virus concentration
Efficient – high transduction efficiency maximises gene delivery into cells
Wide host range – a broad range of mammalian cell types can be transduced
Rapid – expression is much faster than generation of stable cell lines
Interested in BacMam expression? Contact us to find out how we can help.
Our Services
- Validated Protein Standards for Antibody Tests
- Baculovirus Services
- Co-transfection Service
- Titration of Virus Using baculoQUANT™ qPCR
- Virus titration using plaque assay
- Virus safe deposit
- Virus like particle (VLP) production including VLP vaccines
- Amplification of virus inoculum for experimental work or protein production
- BacMam expression service – baculovirus expression in mammalian cells
- Mammalian services
- Project Support
- Optimisation of Expression