Protocols

Quick guide to plaque assays

Quick guide to plaque assays of baculovirus infectivity.  Last week we ran a short training course on baculovirus expression vectors.  As part of this we put together a one page guide for carrying out plaque assays.  This might be useful to those of you looking for something you can have on the lab bench while conducting your own plaque assays.  We have added a image of one of our 12-well

Are they growing? – Beginner’s guide to cell culture

Cell culture may be one of the most routine laboratory tasks, but many times it also proves to be extremely frustrating. Cells grown in the labs are living organisms, and they seem to have the mindset of a moody teenager – happy one moment and extremely upset the very next one. Unfortunately (or fortunately) unlike teenagers they are not able to throw a tantrum and scream out what bothers them.

Cell Culturing OETips, information and manuals

Insect Cell Culture Manual download For Sf9 and Sf21 cell revival guide, please click here For Sf9 cell information, please click here For specialised plaque assay Sf21 information, please click here For Super Sf9 information, please click here How are they growing? Follow our OETips guide to cell culturing here.

Researcher Support, User Manuals, Blog and Tips

Baculovirus and cell culture user guides and protocols: For our Baculovirus protein expression user guide, please click here For Sf9 and Sf21 cell revival guide, please click here For Sf9 cell information, please click here For specialised plaque assay Sf21 information, please click here For Super Sf9 information, please click here Insect Cell Culture Manual download To visit our OET blog for OETips and OETreats click here, or go to oetltd.wordpress.com

baculoFECTIN II and flashFECTIN Protocols and Guides and MSDS

For baculoFECTIN II user guide, please click here For baculoFECTIN II MSDS, please click here For flashFECTIN user guide, please click here For flashFECTIN MSDS, please click here    

List of compatible transfer vectors

As a first choice we recommend the use of our pOET transfer vectors with flashBAC. However other transfer vectors can also be used and here we list other commercially available transfer vectors that are compatible with the flashBAC system.

flashBAC quick start guide to the protocol

The Quick Start guide provides a convenient at a glance guide to protocol for all versions of the flashBAC system. It is very helpful as an at the bench reference guide or as an overview of the essential points surrounding using flashBAC.

BaculoQUANT detailed protocol and background information

If you require detailed information about the baculoQUANT system then this document will be invaluable. It provides background information, detailed protocols, frequently asked questions and a troubleshooting guide. It is highly recommended for first time users.

How flashBAC works

This article provides an introduction to how the flashBAC system works and how to get started.