Cell Culture Passage History and Stress

No, not how you feel when the cells don’t behave! This relates to keeping your cell cultures in a happy state. Cell passage history Each time you sub culture your cells you probably faithfully record on the new flask various bits of information such as the name of the cell line, date, the medium used and passage number.  This last item ideally relates to the number of times the cells

Are they Growing? – Beginner’s Guide to Cell Culture

Cell culture may be one of the most routine laboratory tasks, but many times it also proves to be extremely frustrating. Cells grown in the labs are living organisms, and they seem to have the mindset of a moody teenager – happy one moment and extremely upset the very next one. Unfortunately (or fortunately) unlike teenagers they are not able to throw a tantrum and scream out what bothers them.

Relevant Papers from 2000-2009

2000 King, LA and Possee, RD. (2000). Insect cell culture for baculovirus replication. Encyclopaedia of Life Sciences, Macmillan Press London. In press. Possee,RD and King, LA. (2000). Baculoviruses Encyclopaedia of Life Sciences. Macmillan Press, London. In press. Thomas, CA, Gooday, GW, King, LA and Possee, RD. (2000). Mutagenesis of the active site coding region of the Autographa californica nucleopolyhedrovirus chitinase gene. J. Gen. Virol. 81, 1403-1411. Grasela, JJ., Mcintosh, AH.,

Relevant Papers from 1990-1999

1990 Atkinson, AE, Earley, FPG, Beadle, DJ and King, LA. (1990). Expression and characterization of the chick nicotinic acetylcholine receptor alpha-subunit in insect cells using a baculovirus vector. European Journal of Biochemistry, 192, 451-458. Weitzman, MD, Possee, RD and King, LA. (1990). Virus Insecticides: genetic analysis of Panolis flammea nuclear polyhedrosis virus. In: Pesticides and Alternatives (Ed: Casida, JE) Elsevier Press, Amsterdam, pp. 123-130. Possee, RD, Weyer, U and King,

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