Cell Culture

How to Grow Cells over the Holiday Period

We want to talk about how to grow cells over the holiday period spanning Christmas and New Year.  (OK, so we have covered this before but we think it is worth repetition for new users of the baculovirus-insect cell system).  Most labs, academic and industry, will have some time off over the next few weeks. It is often a problem keeping your cell cultures in good condition so that when

Growing Mammalian Cells Successfully

While OET Ltd has built its reputation on baculovirus-insect cell expression systems, we also undertake work that requires growing mammalian cells successfully too.  Much of this work involves the transduction of these cells with BacMam vectors to express genes placed under the control of mammalian-specific promoter elements.  Some of our work in this area was recently published and involved the generation of improved BacMam vectors for transducing both mammalian cells in

Growing insect cells successfully – Part II

In our previous blog we talked about the early stages of growing insect cells when you might just have revived a frozen stock and set up either monolayer or suspension cultures (or both).  If you have established a viable suspension culture – and here we are talking primarily about insect cells such as Sf21 or Sf9 – you will probably monitor them fairly closely for their rate of growth.  The

Growing Insect Cells Successfully

With Autumn, or Fall as some of you may call it, upon us everyone seems to back in action in the lab again and hopefully growing insect cells successfully.  Regular readers of these blogs will know that we frequently return to the question of how to propagate insect cells.  The reason for doing so is because growing insect cells successfully is fundamental to producing recombinant proteins using the baculovirus system.

Storing Cells at -80°C?

Every so often we are asked if storing insect cells at -80°C is a viable option if you haven’t got access to liquid nitrogen cryogenic facilities.  Our standard response is that this is not a good idea for long term storage of viable cells.  This is largely based on historical dogma that says that cells do not preserve their viability for long at these temperatures.  However, since one of our

When to Passage Sf9 Cells

An important factor in baculovirus expression is assessing when to passage Sf9 cells, which are commonly used to make recombinant viruses or for protein production.  In our last blog we talked about how to make the transition from monolayer to suspension cultures and how maintain cells in a healthy state during this time. To define what is meant by ‘healthy’ cells,  Figure 1 shows a sample of healthy Sf9 cells

Suspension Sf9 Cells from Monolayer Cultures

The transition to suspension Sf9 cells from monolayer cultures is not difficult but does depend on harvesting the attached cells at the right time. Too early and it is extremely hard to detach the cells from the culture flask. Too late and the cells will be in such poor condition that they may not recover when in suspension. Trying to describe in words how the monolayer culture should look prior

Healthy Cells Over the Holiday

We want to talk about maintaining healthy cells over the holiday period spanning Christmas and New Year.  (Some of our regular readers may recognize most of the following from blogs we have posted in previous years, but we think it is worth repetition for new users of the baculovirus-insect cell system). Most labs, academic and industry, will have some time off over the next few weeks. It is often a

Reviving Cells from Liquid Nitrogen

The process of reviving cells from liquid nitrogen, or indeed from transport on dry ice, can be a little difficult on occasion.  Two events at OET recently have highlighted this. Firstly, we received a new cell line that came with a very complicated procedure for its culture – it is a mammalian species, quite different from your run of the mill HeLa’s and HEK293’s.  Its culture is known to be

Adapting Insect Cells to a New Growth Medium?

Have you ever had the experience of adapting insect cells to a new growth medium?  We recently had to do this for a client project because they had used a particular medium previously and were keen to maintain the same conditions to continue the work with us. This process made us realise that switching to a new serum-free insect cell culture medium can result in short term stress responses in

Healthy Culture of Insect Cells

Following on from our last blog on the revival of cells stored in liquid nitrogen, we now provide some tips on the continued healthy culture of insect cells.  Whether you are maintaining cells as monolayer or suspension (shake or spinner) culture you will probably faithfully record your name/medium used/date of sub culture/passage number on the side of your new flask.  Alternatively, instead of using a new flask you may simply

Cryogenic Storage and Revival of Insect Cells

Cryogenic storage of cells is essential to ensure long term, convenient access to new stocks. How well this process is conducted can have a significant impact on the revival process too. In this article we provide a few tips on how both freezing and revival of insect cells can be successfully accomplished.

Expression Systems Media

Expression Systems Insect Cell Culture Media OET is pleased to offer a wide range of Expression Systems Insect cell culture media, from the very popular ESF 921, to specialized insect transfection media. Expression Systems’ media are easy to adapt your cells into and the insect specific media gives great protein yield, when compared to rival media. ESF 921 Insect Culture Media OET use ESF 921 for all in-house work, due

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