Transfection of Insect Cells with Baculovirus DNA

Independent of the method you use to produce recombinant viruses, at some point you have to transfect insect cells with virus DNA to reconstitute infectious virus particles.  When using the flashBAC™ system, you simply add it to your transfer vector containing the foreign gene and use a suitable transfection agent (e.g. baculoFECTIN II )to achieve entry of DNA into the cells.  The transfected cells are then incubated at 28°C for

To Heat Treat or Not to Heat Treat Calf Serum, that is the Question?

Ok, so the Bard is probably spinning in his grave at our blatant plagiarism of the script from the “Scottish Play” (it is bad luck to use its real name, apparently).  However, it seems an apt way to introduce the question of whether or not you should heat treat foetal bovine serum (FBS) before using it to propagate insect cells.  Of course, Spodoptera or Trichoplusia spp. cells are mostly grown

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