superSf9-3 insect cells (cat# 600104) are derived from the Spodoptera frugiperda (Sf9) cell line and are engineered for the enhanced expression of most recombinant proteins. The original Sf9 cell line has become the primary choice for baculovirus expression systems, being used worldwide for the production of recombinant viruses and expresion of proteins. superSf9-3 cells are able to offer a high general expression of a variety of recombinant proteins. This can help to increase the yield of recombinant proteins with mixed characteristics but also those with unknown toxicity and stability. superSf9-3 cells are adapted to serum free suspension culture in Sf-900™ II media but are capable of conforming to other suitable media types. The insect cells can easily be cultured as monolayer cultures in T-flasks, suspension cultures in shake flasks, or scaled up for use in bioreactors.
The cells are prepared from low passage cells (<20 passages in Sf-900™ II medium) and supplied in a cryogenic vial containing 1×107 cells. Freezing medium is comprised of 50% fresh serum free medium, 50% conditioned serum free medium and Dimethyl Sulfoxide (DMSO) to a final concentration of 7.5%.
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