Description

pOET8.VE1 (cat# 200121) (10µg) is a baculovirus transfer vector designed for high level expression of foreign genes under the powerful AcMNPV polyhedrin gene (polh) promoter. Derived from the pUC57 vector, it contains a vankyrin expression cassette, P-vank-1¹, which encodes an anti-apoptotic protein to help delay cell death following virus infection. It has a bacterial origin of replication and an ampicillin resistance gene for selection in E. coli whilst the polh coding sequences have been replaced by a multiple cloning site (MCS) containing unique restriction enzyme sites for insertion of the foreign gene in the correct orientation. pOET8.VE1 is compatible with any baculovirus expression system that utilizes homologous recombination in insect cells.

¹ Fath-Goodin, A., Kroemer, J.A., Martin, S.B., Reeves, K., and Webb, B.A. (2006). Polydnavirus genes that enhance the Baculovirus Expression Vector System. Advances in Virus Research, vol. 68, pp. 75-90. Click here for link.

Variations

• pOET8.VE2 has an additonal N-terminal 8xHis-Tag^® and Honey Bee Melittin signal sequence
• pOET8.VE3 has an additional C-terminal 6xHis-Tag^® and Honey Bee Melittin signal sequence