pOET6 BacMAM Gateway™ Vector

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pOET6 BacMAM Gateway Box
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pOET6 BacMAM Gateway™ Vector

£325.00 exc. VAT

The pOET6 BacMAM Gateway™ vector combines the rapid and easy to use gene shuttling technology developed by Life Technologies Corporation with the one-step and highly efficient flashBAC™ recombinant protein expression system.

Product Description

pOET6 BacMAM Gateway ™ (cat# 200109) (6ug) is a baculovirus transfer vector designed for high level expression of foreign genes in mammalian cell lines, under the powerful cytomegalovirus (CMV) immediate early gene promoter. Using this powerful promoter allows for genes to be expressed in mammalian cell lines (e.g. CHO and HEK) but prevents the baculovirus from replicating, thus making it one of the safest mammalian protein expression systems on the market. The vector has a cassette with the ccdB gene (The CcdB protein inhibits growth of most E. coli strains by interfering with DNA gyrase) and chloramphenicol resistance gene between two different attR sites, which enable LR recombination to replace the cassette with the gene of interest from an entry vector in the correct orientation. It also contains a bacterial origin of replication and an ampicillin resistance gene for selection in E. coli (E. coli strain must be resistant to CcdB effects, such as DB3.1™). The AcMNPV sequences flanking the gene in the transfer vectors MCS allow recombination with the viral DNA to insert the expression cassette into the polh locus. pOET6 BacMAM Gateway ™ is compatible with any baculovirus system that utilizes homologous recombination in insect cells.

Variations

  • pOET6 BacMAM is the original transfer vector designed for mammalian cell expression that does not combine the Gateway ™ Technology