Description

pOET3 (cat# 200104) (10ug) is a baculovirus transfer vector designed for high level expression of foreign genes under the late AcMNPV basic (p6.9) promoter. Using this promoter will provide earlier expression compared to the polyhedrin promoter. This has been shown to be beneficial when expressing proteins which require extensive post ­translational modifications i.e. glycosylation. The vector is smaller than other available transfer vectors (4530bp) which greatly facilitates the cloning steps. It has a ColE1 origin of replication and an ampicillin resistance gene for selection in E. coli. The polyhedrin sequences have been replaced by a multiple cloning site (MCS) containing unique restriction sites for insertion of the foreign gene in the correct orientation. The AcMNPV sequences flanking the gene in the transfer vector’s MCS allow recombination with the viral DNA to insert the expression cassette into the polh locus. pOET3 is compatible with any baculovirus system that utilises homologous recombination in insect cells.

Variations

• pOET4 has the same MCS as pOET3 but in the reverse orientation.