pOET2.1 (cat# 200103) (10ug) is a baculovirus transfer vector designed for high level expression of foreign genes under the powerful AcMNPV polyhedrin (polh) promoter. The vector is smaller than other available transfer vectors (4575bp) which greatly facilitates the cloning steps. It has a ColE1 origin of replication and an ampicillin resistance gene for selection in E. coli. The polh sequences have been replaced by a multiple cloning site (MCS) in the reverse orientation to pOET1.1, containing unique restriction sites for insertion of the foreign gene. The AcMNPV sequences flanking the gene in the transfer vector’s MCS allow recombination with the viral DNA to insert the expression cassette into the polh locus. pOET2.1 is compatible with any baculovirus system that utilises homologous recombination in insect cells.
- pOET1.1 has the same MCS as pOET2.1 but in the reverse orientation.