pOET2 Transfer Plasmid

pOET2 Box
pOET Tube

pOET2 Transfer Plasmid

£139.00 exc. VAT

Product Description

pOET2 (cat# 200103) (10ug) is a baculovirus transfer vector designed for high level expression of foreign genes under the powerful AcMNPV polyhedrin (polh) promoter.  The vector is smaller than other available transfer vectors (4547bp) which greatly facilitates the cloning steps. It has a ColE1 origin of replication and an ampicillin resistance gene for selection in E. coli. The polh sequences have been replaced by a multiple cloning site (MCS) in the reverse orientation to pOET1, containing unique restriction sites for insertion of the foreign gene. The AcMNPV sequences flanking the gene in the transfer vector’s MCS allow recombination with the viral DNA to insert the expression cassette into the polh locus. pOET2 is compatible with any baculovirus system that utilises homologous recombination in insect cells.

Variations

pOET1 has the same MCS as pOET2 but in the reverse orientation.

 

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.