pOET1C 6xHis Transfer Plasmid

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pOET1C 6xHis Box
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pOET1C 6xHis Transfer Plasmid

£145.00 exc. VAT

Product Description

pOET1C 6xHis (cat# 2001012) (10ug) is a baculovirus transfer vector designed for high level expression of foreign genes under the powerful AcMNPV polyhedron (polh) promoter.  The vector encodes an optional C-terminal 6xHis-Tag® fusion sequence that may be utilised.  This greatly eases the purification of the recombinant protein since the 6xHis-containing fusion proteins bind with high affinity to Ni-NTA Agarose.  pOET1C has a ColE1 origin of replication and an ampicillin resistance gene for selection in E. coli. The polh sequences have been replaced by a multiple cloning site (MCS) containing unique restriction sites for insertion of the foreign gene in the correct orientation.  The AcMNPV sequences flanking the gene in the transfer vector’s MCS allow recombination with the viral DNA to insert the expression cassette into the polh locus. pOET1C is compatible with any baculovirus system that utilises homologous recombination in insect cells.

Variations

  • pOET2C 6xHis has the same MCS as pOET1C 6xHis except in the reverse orientation.
  • pOET1N 6xHis has an N-terminal rather than C-terminal 6xHis-Tag®.