The flashBAC selection box (cat# 100400/100401) enables you to try out all variations of the flashBAC™ technology in one convenient kit. flashBAC™ vectors are the easiest and quickest baculovirus protein expression system available, with a choice of gene deletions from the baculovirus genome designed to suit the production of different proteins.
Each flashBAC selection box contains either a single 3 reaction kit of flashBAC, flashBAC GOLD and flashBAC ULTRA (Box 1) or a single 3 reaction kit of flashBAC, flashBAC GOLD, flashBAC ULTRA and flashBAC PRIME (Box 2). The original flashBAC expression vector is a good choice for projects where the gene to be expressed is likely to be simple and expressed in the cytoplasm or nucleus. flashBAC GOLD is enhanced by the deletion of the chiA (chitinase) and v-cath (cathepsin) protease genes. It provides superior levels of expression for any protein that is destined for secretion or to be inserted in the membrane, or for any protein that might be particularly liable to degradation. flashBAC ULTRA contains deletions of the chitinase and cathepsin genes, as above, but also deletions of the p10, p74 and p26 genes and provides the best choice for the most difficult to express proteins; suitable for cytoplasmic, nucleus, secreted and membrane proteins. flashBAC PRIME contains no gene deletions and has proven useful for the release and subsequent purification of intracellular VLPs and protein complexes.