Description
baculoQUANT™ ALL-IN-ONE (Cat# 100602) is the revolutionary qPCR based baculovirus DNA extraction and quantification kit from OET’s collaboration with Agilent Technologies. Accurate virus titres are essential for determining correct multiplicity of infection and to ensure optimal recombinant protein production. Traditionally, baculoviruses have been titrated by plaque assays or antibody based assays that require 3-4 days, however the baculoQUANT™ ALL-IN-ONE kit contains reagents to extract viral DNA and determine accurate titres within hours.
Benefits of using baculoQUANT™ ALL-IN-ONE:
- Titre accuracy comparable to plaque assay method
- Less than one hour hands-on time with virus DNA extraction and titration in under three hours
- Compatible with any AcMNPV based baculovirus system containing gp64 virus gene
- Single step virus DNA extraction without the need for columns
- qPCR primers/probe provided as a single reagent mix
- Agilent Technologies’ high performance, ultra sensitive master mix reagent
- Plaque assay titrated virus stocks included for standard curve generation
- 100 reaction kit allows for up to 24 titrations in triplicate with 3 standard curves
