baculoCOMPLETE with baculoQUANT™ ALL-IN-ONE (cat# 400101) is the ultimate baculovirus expression package. By combining two of our most popular products you can conveniently carry out recombinant virus production and quantification in one go. baculoCOMPLETE is the perfect choice for those new to the baculovirus expression systems or for those wanting to upgrade to the flashBAC™ expression platform. All items within the kit have been optimised to complement one another and allow you to produce the best titres of recombinant virus and protein yield. Meanwhile, baculoQUANT™ ALL-IN-ONE is the revolutionary qPCR based baculovirus DNA extraction and quantification kit from OET’s collaboration with Agilent Technologies. Traditionally, baculoviruses have been titrated by plaque assays or antibody based assays that require 3-4 days, however the baculoQUANT™ ALL-IN-ONE kit contains reagents to extract viral DNA and determine accurate titres within hours.

baculoCOMPLETE with baculoQUANT™ ALL-IN-ONE kit contains:

  • flashBAC ULTRA DNA 5 reaction kit (500ng/µl)
  • Control transfer vector DNA (expressing lacZ under polyhedrin (polh) gene promoter) (500ng/µl)
  • baculoFECTIN II transfection reagent (50µl)
  • ESF 921 insect cell media (1L)
  • Transfection media (50ml)
  • Insect Sf9 cells frozen (1 x 10cells)
  • baculoQUANT™ ALL-IN-ONE virus extraction and titration kit (100 reactions)

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