The original Sf9 cell line has become the primary choice for baculovirus expression systems, being used worldwide for the production of recombinant viruses and expresion of proteins. superSf9-1 cells are able to offer a prolonged expression time following baculovirus infection. This can help to increase the yield of more stable intracellular, membrane and secreted proteins by up to 8-fold, provided that they are not subject to degredation. superSf9-1 cells are adapted to serum free suspension culture in Sf-900™ II media but are capable of conforming to other suitable media types. The insect cells can easily be cultured as monolayer cultures in T-flasks, suspension cultures in shake flasks, or scaled up for use in bioreactors.