Transfer Plasmids

OET's range of baculovirus transfer vectors are designed for high level expression of foreign genes in both insect and mammalian cell lines. Our pOET transfer plasmids are ideal for use with the flashBAC™ and BacPAK6 baculovirus expression systems but are also fully compatible with other homologous recombination technologies including: BacMagic™ (EMD Millipore) BacMagic2™ (EMD Millipore) BacMagic3™ (EMD Millipore) BaculoGOLD Bright™ (BD Bioscience) BacPAK6 ProGreen™ (AbVector) BacPAK6 ProFold™ (AbVector) BacPAK6 ProEasy™ (AbVector) Sapphire™ (Orbigen) In addition, OET is also happy to announce the pOET Gateway™ vector range that combines the fast and easy to use Gateway™ technology (Life Technologies), with the one-step, rapid, high-throughput flashBAC™ system.

pOET Transfer Plasmids

OET's range of pOET transfer plasmids are designed for high level expression of foreign genes under the powerful AcMNPV polyhedrin (polh), p10, p6.9 or CMV gene promoters. pOET plasmids are smaller than other available transfer vectors which greatly facilitates cloning steps. Each vector has a bacterial origin of replication and an ampicillin resistance gene for selection in E. coli whilst the MCS has numerous unique restriction enzyme sites for insertion of the foreign gene in the correct orientation(s).

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