Transfer Plasmids

OET's range of baculovirus transfer vectors are designed for high level expression of foreign genes in both insect and mammalian cell lines. Our pOET transfer plasmids are ideal for use with the flashBAC™ and BacPAK6 baculovirus expression systems but are also fully compatible with other homologous recombination technologies including: BacMagic™ (EMD Millipore) BacMagic2™ (EMD Millipore) BacMagic3™ (EMD Millipore) BaculoGOLD Bright™ (BD Bioscience) BacPAK6 ProGreen™ (AbVector) BacPAK6 ProFold™ (AbVector) BacPAK6 ProEasy™ (AbVector) Sapphire™ (Orbigen) In addition, OET is also happy to announce the pOET Gateway™ vector range that combines the fast and easy to use Gateway™ technology (Life Technologies), with the one-step, rapid, high-throughput flashBAC™ system.

Gateway™ Transfer Vectors

The pOET Gateway ™ vectors for flashBAC™ are combined to make insertion of your target gene easier and quicker. Our patented flashBAC™ technology provides the convenience of a one-step process for inserting foreign genes into Baculovirus genomes, whilst the Life Technologies Corporation Gateway ™ vector offers rapid and easy to use gene shuttling technology. Until now it has been necessary to produce a transfer plasmid containing the gene target using traditional ligation techniques with our pOET range of vectors. However, we are now pleased to offer the convenience of the Gateway™ vector cloning technology to flashBAC™ users.

pOET Transfer Plasmids

OET's range of pOET transfer plasmids are designed for high level expression of foreign genes under the powerful AcMNPV polyhedrin (polh), p10, p6.9 or CMV gene promoters. pOET plasmids are smaller than other available transfer vectors which greatly facilitates cloning steps. Each vector has a bacterial origin of replication and an ampicillin resistance gene for selection in E. coli whilst the MCS has numerous unique restriction enzyme sites for insertion of the foreign gene in the correct orientation(s).

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