We have a comprehensive range of products to support successful gene expression and protein production in insect or mammalian cells! This includes a range of baculovirus expression kits based on flashBAC, GOLD, ULTRA and PRIME. We also provide kits based on linear DNA technology - BacPAK6.
It is essential that an accurate titre of virus be obtained to determine correct multiplicity of infection and to ensure optimal recombinant protein production. Traditionally, baculoviruses have been titrated by plaque assays or antibody based assays that requires 3-4 days. OET has teamed up with Agilent Technologies to introduce a revolutionary qPCR-based, baculovirus titration kit - baculoQUANT™ ALL-IN-ONE. By utilising qPCR, it is possible to accurately establish viral titres from large numbers of budded virus stocks in a number of hours. The kit includes all the components required to extract and quantify viral DNA. We also offer baculoQUANT™ ALL-IN-ONE as part of a package with baculoCOMPLETE so that you can conveniently carry out recombinant virus production and titration in one go.
OET provides a range of baculovirus expression kits based on flashBAC, GOLD, ULTRA and PRIME. We also provide kits based on linear DNA technology - BacPAK6.
pOET Transfer Plasmids
OET’s range of baculovirus transfer vectors are designed for high level expression of foreign genes in both insect and mammalian cell lines. Our pOET transfer plasmids are ideal for use with the flashBAC™ and BacPAK6 baculovirus expression systems but are also fully compatible with other homologous recombination technologies including: BacMagic™ (EMD Millipore) BacMagic2™ (EMD Millipore) BacMagic3™ (EMD Millipore) BaculoGOLD Bright™ (BD Bioscience) BacPAK6 ProGreen™ (AbVector) BacPAK6 ProFold™ (AbVector) BacPAK6 ProEasy™ (AbVector) Sapphire™ (Orbigen)
baculoFECTIN II is a novel nanoparticle-based transfection reagent specifically developed and optimised for the transfection of insect cells. Non-toxic, simple to use, and efficient, baculoFECTIN II transfection reagent is an excellent choice for producing high titre recombinant virus stocks whilst maintaining cell health. Our transfection reagent is compatible with multiple cell lines and baculovirus expression vectors includuing our own flashBAC™ and BacPAK6 technology.