The flashBAC™ system is a revolutionary platform technology for the production of recombinant baculoviruses in insect cells. It comprises a parental AcMNPV genome that lacks part of an essential gene (ORF 1629), which removes the need to separate recombinant virus from parental virus. We offer several different types of flashBAC™, each with different set of deletions to enhance the yield of recombinant proteins. Yes, also those difficult ones that you failed to express using other methods!
flashBAC™ – the original version of flashBAC™. Great for expression of simple, nucleus- or cytoplasm-targeted proteins.
flashBAC GOLD – provides superior levels of expression for any protein that is destined for secretion or to be inserted in the membrane, or for any protein that might be particularly liable to degradation. Enhanced levels of expression of these proteins are due to chiA deletion which improves the efficacy of secretory pathway, and v-cath deletion which contributes to the reduction of protein degradation rates.
flashBAC ULTRA – the ultimate version of flashBAC™. In addition to chiA and v-cath, three more virus genes (p10, p74 and p26) have been deleted from the flashBAC ULTRA genome. This has allowed further improvements in recombinant protein yield and quality to be delivered, making flashBAC ULTRA™ the best choice for the most difficult to express proteins.
flashBAC PRIME – contains no gene deletions and has proven useful for the release and subsequent purification of intracellular VLPs and protein complexes.
The recipe to create your very own baculovirus using flashBAC™ kit is simple: prepare the co-transfection mix of flashBAC™ DNA, transfer vector containing the gene of interest and transfection reagent, use it to transfect healthy insect cells, incubate for 5 days et voila – the baculovirus is served. It may then be amplified and used for experimental work.
The simplicity of this process makes it perfect for both the beginners only starting their adventure with baculoviruses, as well as those with several years of experience wishing to enhance their results or upscale the production.
So why don’t you have a go and try it yourself?