News Stories

Plaque-Assay Schematic

We’ve said it enough times; the plaque-assay is the ‘gold standard’ for determining virus infectivity. But this doesn’t make the procedure any easier! Whether it’s the agonising 5 day waiting period or the disorientating number of steps, plaque-assays can seem like a chore and we’ve heard of more than enough non-virologists admit to skipping this laborious technique in favour of a simpler solution. More often than not this isn’t a

Impact of Brexit

As you are aware the UK is due to leave the EU on 29th March 2019. Negotiations are still ongoing so it is yet unclear how this will affect trade going in and out of the UK. OET will be preparing for all possible outcomes as best we can to ensure that customer orders can still be fulfilled. However, we recommend that if you know you will require OET products

Growing Mammalian Cells Successfully

While OET Ltd has built its reputation on baculovirus-insect cell expression systems, we also undertake work that requires growing mammalian cells successfully too.  Much of this work involves the transduction of these cells with BacMam vectors to express genes placed under the control of mammalian-specific promoter elements.  Some of our work in this area was recently published and involved the generation of improved BacMam vectors for transducing both mammalian cells in

baculoQUANT™ Titration of Recombinant Baculoviruses

There are many methods available for the titration of recombinant baculoviruses.  One of the most convenient is the use of the quantitative polymerase chain reaction (QPCR), which assesses how much virus DNA is present in a freshly-amplified stock.  This is compared with a standard curve derived from a control virus stock to give an infectious titre expressed as Q plaque forming units (pfu) per millilitre.  OET Ltd market the baculoQUANT™

Expression Systems Media

Expression Systems Insect Cell Culture Media OET is pleased to offer a wide range of Expression Systems Insect cell culture media, from the very popular ESF 921, to specialized insect transfection media. Expression Systems’ media are easy to adapt your cells into and the insect specific media gives great protein yield, when compared to rival media. ESF 921 Insect Culture Media OET use ESF 921 for all in-house work, due