News Stories

PhD Studentship

3.5 year, full-time PhD studentship Project title: Baculovirus P10 and microtubules; the role of P10 in virus-induced nuclear lysis with possible applications for novel anti-cancer therapies Eligibility: Home UK/EU applicants who must be permanently resident in the UK/EU Closing date: 20 March 2020 Start date: September 2020 Bursary p.a.: equivalent to UKRI national minimum stipend plus fees (2019/20 rate is £15,009) University fees and bench fees at the Home/EU rate

Introduction to the Baculovirus Expression System

Baculoviruses are in many respects an odd choice for use as an expression system to make recombinant proteins.  In their natural environment they infect insect larvae/caterpillars, which frequently results in the death of the host and its reduction to a puddle of progeny virus (Fig. 1).  They are also unusual in that they produce two structurally different forms in their life cycle. The first is a lipoprotein-enveloped particle that buds

baculoQUANT™ ALL-IN-ONE Titration of Recombinant Baculoviruses

There are many methods available for the titration of recombinant baculoviruses.  One of the most convenient is the use of the quantitative polymerase chain reaction (qPCR), which assesses how much virus DNA is present in a freshly-amplified stock.  This is compared with a standard curve derived from a control virus stock to give an infectious titre expressed as Q plaque forming units (pfu) per millilitre.  OET Ltd market the baculoQUANT™