News Stories

Culturing Insect Cells

On a scale of complexity/difficulty, culturing insect cells falls somewhere between yeast and mammalian systems. They can be grown in suspension or as monolayer cultures.  However, the latter format does not require trypsin enzyme treatment prior to harvest and sub culture or passage.  Further, the growth medium does not require the use of carbon dioxide to regulate the pH as is the case in many mammalian monolayer cultures.  Unlike yeast,

Selecting a Protein Expression System

A major decision facing anyone starting a new project requiring any quantity of recombinant protein is selecting a protein expression system. A quick search online will unearth a large number of options and an often bewildering choice of different vectors to use within any given system. For the novice, this can be daunting! It may sound obvious, but the first thing to do is to decide what you want your

Expression Systems Media

Expression Systems Insect Cell Culture Media OET is pleased to offer a wide range of Expression Systems Insect cell culture media, from the very popular ESF 921, to specialized insect transfection media. Expression Systems’ media are easy to adapt your cells into and the insect specific media gives great protein yield, when compared to rival media. ESF 921 Insect Culture Media OET use ESF 921 for all in-house work, due

New pOET9 Vectors!

pOET9 transfer vectors from OET incoporate baculovirus technology with unique mammalian promoters to facilitate protein production in mammalian cell lines.