Transduction of Whole Porcine Kidneys with BacMams
One of the unexpected offshoots of the baculovirus expression system has been its development as a vehicle for the delivery of genes to...
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EXPERTS IN BACULOVIRUS PROTEIN EXPRESSION
BAC TO THE FUTURE
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Nov 30, 20162 min read
Healthy Cells Over the Holiday
For a follow up to our last blog we want to talk about maintaining healthy cells over the holiday period spanning Christmas and New Year....
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Nov 22, 20164 min read
Healthy Culture of Insect Cells
Following on from our last blog on the revival of cells stored in liquid nitrogen, we now provide some tips on the continued healthy...
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Oct 14, 20162 min read
Insoluble Proteins – Inconsolable!
You may have had the experience! An expression project is going really well. You inserted your gene into a baculovirus vector...
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Sep 22, 20163 min read
Protein Expression and Directed Mutations
We get asked to do many service projects for customers where they want to produce a modified form of a protein for structural/functional...
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Aug 18, 20163 min read
Louis Pasteur and Insect Viruses
We told you last time about our trip to the Society for Invertebrate Pathology meeting in Tours, France. This was another highly...
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Jul 22, 20162 min read
Bac to Our Roots!
Ever wondered where the baculovirus insect cell expression system came from? The clue is in the name – of course it came from viruses...
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Jul 14, 20163 min read
Baculovirus Gene Mutations and Protein Expression
Have you ever wondered why the baculovirus we use for most protein expression purposes is called AcMNPV? This is short for Autographa...
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Jun 29, 20163 min read
Producing Virus-Like Particles (VLPs) in Insect Cells
Using baculovirus expression vectors to produce virus-like particles (VLPs) is not new. Intact, non-infectious poliovirus1 and...
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Jun 14, 20163 min read
Producing Secreted Proteins
We had promised to consider the production of virus like particles in this blog, but have postponed this topic to a later date after the...
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May 31, 20163 min read
Protein Purification
After scaling up recombinant baculovirus-insect cell cultures (blog) we now come to what can be a very tricky part of the operation,...
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May 13, 20164 min read
Scaling Up Protein Production
Once you have decided on the optimal conditions for producing your recombinant protein target in baculovirus-infected insect cells, your...
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Apr 26, 20163 min read
Optimising Protein Expression
Once you have finished making your recombinant baculoviruses expression vector you naturally want to maximize the amount of protein made...
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Apr 11, 20163 min read
Longevity of Infectious Budded Baculovirus Stocks
A question that comes up repeatedly is the long term stability of recombinant budded baculovirus stocks. This is a particular issue now...
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Mar 31, 20163 min read
Rapid Virus Titration by QPCR
Knowing the infectious titre of a recombinant virus stock is really important prior to testing for protein production in insect cells. ...
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Mar 11, 20164 min read
Titrating Stocks of Recombinant Baculoviruses Using the Plaque Assay Technique
We were going to devote this blog to a comprehensive account of how you might determine the infectious titre of your virus stock. ...
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Feb 25, 20165 min read
Production of High Titre Virus Stocks
In our last blog post we talked about the process of transfecting insect cells with virus DNA and how you could recognise success or...
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Feb 10, 20163 min read
Transfection of Insect Cells with Baculovirus DNA
Independent of the method you use to produce recombinant viruses, at some point you have to transfect insect cells with virus DNA to...
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Jan 29, 20162 min read
How healthy are your cells?
Hopefully after the seasonal break your insect cells are still in good health. Recognizing healthy cells seems a no brainer, but if you...
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Jan 13, 20162 min read
To heat treat or not to heat treat calf serum, that is the question?
Ok, so the Bard is probably spinning in his grave at our blatant plagiarism of the script from the “Scottish Play” (it is bad luck to use...
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