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EXPERTS IN BACULOVIRUS PROTEIN EXPRESSION

BAC TO THE FUTURE


Selecting a Protein Expression System
A major decision facing anyone starting a new project requiring any quantity of recombinant protein is selecting a protein expression...

yelluk
Oct 9, 20193 min read
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Pre-GMP Manufacture of Recombinant Proteins
While OET is currently unable to offer GMP manufacture of recombinant proteins or viruses, we can offer a premium service for pre-GMP...

yelluk
Jun 12, 20182 min read
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Co-infection with Baculovirus Vectors for Multiple Protein Expression
A question that comes up from time to time is the use of co-infection with baculovirus vectors for multiple protein expression in insect...

yelluk
Oct 4, 20172 min read
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Variable Expression of Multi-Component Protein Complexes
Variable expression of multi-component protein complexes is increasingly necessary as more sophisticated projects are attempted. But how...

yelluk
Sep 8, 20172 min read
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Baculovirus Expressed Secreted Recombinant Proteins
A question that often comes up from clients is what is the best signal peptide to use for baculovirus-expressed secreted recombinant...
yelluk
May 30, 20172 min read
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Insoluble Proteins – Inconsolable!
You may have had the experience! An expression project is going really well. You inserted your gene into a baculovirus vector...
yelluk
Oct 14, 20162 min read
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Protein Expression and Directed Mutations
We get asked to do many service projects for customers where they want to produce a modified form of a protein for structural/functional...
yelluk
Sep 22, 20163 min read
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Baculovirus Gene Mutations and Protein Expression
Have you ever wondered why the baculovirus we use for most protein expression purposes is called AcMNPV? This is short for Autographa...
yelluk
Jul 14, 20163 min read
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Producing Secreted Proteins
We had promised to consider the production of virus like particles in this blog, but have postponed this topic to a later date after the...
yelluk
Jun 14, 20163 min read
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Protein Purification
After scaling up recombinant baculovirus-insect cell cultures (blog) we now come to what can be a very tricky part of the operation,...
yelluk
May 31, 20163 min read
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Scaling Up Protein Production
Once you have decided on the optimal conditions for producing your recombinant protein target in baculovirus-infected insect cells, your...
yelluk
May 13, 20164 min read
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Optimising Protein Expression
Once you have finished making your recombinant baculoviruses expression vector you naturally want to maximize the amount of protein made...
yelluk
Apr 26, 20163 min read
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To heat treat or not to heat treat calf serum, that is the question?
Ok, so the Bard is probably spinning in his grave at our blatant plagiarism of the script from the “Scottish Play” (it is bad luck to use...
yelluk
Jan 13, 20162 min read
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