yelluk
Selecting a Protein Expression System
A major decision facing anyone starting a new project requiring any quantity of recombinant protein is selecting a protein expression...
top of page
EXPERTS IN BACULOVIRUS PROTEIN EXPRESSION
BAC TO THE FUTURE
yelluk
Jun 12, 20182 min read
Pre-GMP Manufacture of Recombinant Proteins
While OET is currently unable to offer GMP manufacture of recombinant proteins or viruses, we can offer a premium service for pre-GMP...
24 views0 comments
yelluk
Oct 4, 20172 min read
Co-infection with Baculovirus Vectors for Multiple Protein Expression
A question that comes up from time to time is the use of co-infection with baculovirus vectors for multiple protein expression in insect...
21 views0 comments
yelluk
Sep 8, 20172 min read
Variable Expression of Multi-Component Protein Complexes
Variable expression of multi-component protein complexes is increasingly necessary as more sophisticated projects are attempted. But how...
6 views0 comments
yelluk
May 30, 20172 min read
Baculovirus Expressed Secreted Recombinant Proteins
A question that often comes up from clients is what is the best signal peptide to use for baculovirus-expressed secreted recombinant...
15 views0 comments
yelluk
Oct 14, 20162 min read
Insoluble Proteins – Inconsolable!
You may have had the experience! An expression project is going really well. You inserted your gene into a baculovirus vector...
14 views0 comments
yelluk
Sep 22, 20163 min read
Protein Expression and Directed Mutations
We get asked to do many service projects for customers where they want to produce a modified form of a protein for structural/functional...
10 views0 comments
yelluk
Jul 14, 20163 min read
Baculovirus Gene Mutations and Protein Expression
Have you ever wondered why the baculovirus we use for most protein expression purposes is called AcMNPV? This is short for Autographa...
48 views0 comments
yelluk
Jun 14, 20163 min read
Producing Secreted Proteins
We had promised to consider the production of virus like particles in this blog, but have postponed this topic to a later date after the...
18 views0 comments
yelluk
May 31, 20163 min read
Protein Purification
After scaling up recombinant baculovirus-insect cell cultures (blog) we now come to what can be a very tricky part of the operation,...
12 views0 comments
yelluk
May 13, 20164 min read
Scaling Up Protein Production
Once you have decided on the optimal conditions for producing your recombinant protein target in baculovirus-infected insect cells, your...
16 views0 comments
yelluk
Apr 26, 20163 min read
Optimising Protein Expression
Once you have finished making your recombinant baculoviruses expression vector you naturally want to maximize the amount of protein made...
22 views0 comments
yelluk
Jan 13, 20162 min read
To heat treat or not to heat treat calf serum, that is the question?
Ok, so the Bard is probably spinning in his grave at our blatant plagiarism of the script from the “Scottish Play” (it is bad luck to use...
5 views0 comments
bottom of page