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EXPERTS IN BACULOVIRUS PROTEIN EXPRESSION
BAC TO THE FUTURE
yelluk
Dec 18, 20193 min read
The Cells at Christmas Conundrum
As the holiday season creeps painfully slowly towards us it’s not only the lab staff who need a little TLC over the festive break. Cell...
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yelluk
Oct 24, 20193 min read
Insect Cell Culture Media
Following on from our last blog we briefly describe insect cell culture media. It used to be that all animal cell cultures depended on...
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yelluk
Oct 16, 20193 min read
Culturing Insect Cells
On a scale of complexity/difficulty, culturing insect cells falls somewhere between yeast and mammalian systems. They can be grown in...
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yelluk
Dec 18, 20183 min read
How to Grow Cells over the Holiday Period
We want to talk about how to grow cells over the holiday period spanning Christmas and New Year. (OK, so we have covered this before but...
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yelluk
Nov 26, 20183 min read
Growing Mammalian Cells Successfully
While OET Ltd has built its reputation on baculovirus-insect cell expression systems, we also undertake work that requires growing...
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yelluk
Nov 13, 20183 min read
Growing insect cells successfully – Part II
In our previous blog we talked about the early stages of growing insect cells when you might just have revived a frozen stock and set up...
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yelluk
Oct 12, 20183 min read
Growing Insect Cells Successfully
With Autumn, or Fall as some of you may call it, upon us everyone seems to back in action in the lab again and hopefully growing insect...
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yelluk
Mar 27, 20182 min read
Storing Insect Cells at -80°C?
Every so often we are asked if storing insect cells at -80°C is a viable option if you haven’t got access to liquid nitrogen cryogenic...
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yelluk
Mar 6, 20182 min read
When to Passage Sf9 Cells
An important factor in baculovirus expression is assessing when to passage Sf9 cells, which are commonly used to make recombinant viruses...
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yelluk
Feb 22, 20183 min read
Suspension Sf9 Cells from Monolayer Cultures
The transition to suspension Sf9 cells from monolayer cultures is not difficult but does depend on harvesting the attached cells at the...
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yelluk
Nov 24, 20172 min read
Reviving Cells from Liquid Nitrogen
The process of reviving cells from liquid nitrogen, or indeed from transport on dry ice, can be a little difficult on occasion. Two...
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yelluk
Jun 21, 20172 min read
Adapting Insect Cells to a New Growth Medium?
Have you ever had the experience of adapting insect cells to a new growth medium? We recently had to do this for a client project...
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yelluk
Feb 1, 20172 min read
Sf9 Cells from Monolayers to Suspension Cultures
Transferring Sf9 cells from monolayers to suspension cultures can sometimes be difficult. These insect cells, commonly employed for...
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yelluk
Nov 30, 20162 min read
Healthy Cells Over the Holiday
For a follow up to our last blog we want to talk about maintaining healthy cells over the holiday period spanning Christmas and New Year....
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yelluk
Nov 22, 20164 min read
Healthy Culture of Insect Cells
Following on from our last blog on the revival of cells stored in liquid nitrogen, we now provide some tips on the continued healthy...
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yelluk
Jan 29, 20162 min read
How healthy are your cells?
Hopefully after the seasonal break your insect cells are still in good health. Recognizing healthy cells seems a no brainer, but if you...
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yelluk
Dec 21, 20152 min read
Cells at Christmas
So, you have had the Christmas Party and hopefully are looking forward to the holiday season. Many labs shut down between Christmas and...
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yelluk
Dec 16, 20152 min read
Cell Culture Passage history and stress
No, not how you feel when the cells don’t behave! This relates to keeping your cell cultures in a happy state. 1. Cell passage history...
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yelluk
Dec 8, 20152 min read
Are They Growing? – Beginner’s guide to Cell Culture
Cell culture may be one of the most routine laboratory tasks, but many times it also proves to be extremely frustrating. Cells grown in...
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