yelluk
Plaque-Assay Schematic
We’ve said it enough times; the plaque-assay is the ‘gold standard’ for determining virus infectivity. But this doesn’t make the...
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EXPERTS IN BACULOVIRUS PROTEIN EXPRESSION
BAC TO THE FUTURE
yelluk
Nov 13, 20183 min read
Growing insect cells successfully – Part II
In our previous blog we talked about the early stages of growing insect cells when you might just have revived a frozen stock and set up...
106 views0 comments
yelluk
Jul 9, 20182 min read
Baculovirus Transfer Vector Plasmid Compatibility
A frequent customer query concerns baculovirus transfer vector plasmid compatibility with either flashBACTM or other systems for making...
48 views0 comments
yelluk
Jun 12, 20182 min read
Pre-GMP Manufacture of Recombinant Proteins
While OET is currently unable to offer GMP manufacture of recombinant proteins or viruses, we can offer a premium service for pre-GMP...
24 views0 comments
yelluk
Oct 4, 20172 min read
Co-infection with Baculovirus Vectors for Multiple Protein Expression
A question that comes up from time to time is the use of co-infection with baculovirus vectors for multiple protein expression in insect...
21 views0 comments
yelluk
Sep 20, 20172 min read
Baculovirus Transfer Vectors Compatible with flashBAC™
A question we are asked from time to time is to identify baculovirus transfer vectors compatible with our flashBAC™ system. We obviously...
33 views0 comments
yelluk
Sep 8, 20172 min read
Variable Expression of Multi-Component Protein Complexes
Variable expression of multi-component protein complexes is increasingly necessary as more sophisticated projects are attempted. But how...
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yelluk
Jul 25, 20171 min read
Intermediate scale recombinant protein production
Between initial tests and larger scale up you may want to conduct intermediate scale recombinant protein production using baculovirus...
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yelluk
Jun 22, 20171 min read
Welcome!
Oxford Expression Technologies are the experts when it comes to the science of baculovirus. For almost 10 years now we have specialised...
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yelluk
May 30, 20172 min read
Baculovirus Expressed Secreted Recombinant Proteins
A question that often comes up from clients is what is the best signal peptide to use for baculovirus-expressed secreted recombinant...
16 views0 comments
yelluk
May 2, 20173 min read
baculoQUANT Titration of Recombinant Baculoviruses
There are many methods available for the titration of recombinant baculoviruses. One of the most convenient is the use of the...
28 views0 comments
yelluk
Apr 26, 20172 min read
Sequence Errors in Your Gene for Expression Part II?
Sequence errors in your gene for expression Part II follows on from an earlier blog where we discussed a problem we were having producing...
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yelluk
Apr 3, 20173 min read
Sequence Errors in Your Gene for Expression?
If you don’t achieve the levels of protein expression expected in your project, have you ever considered that there may be sequence...
15 views0 comments
yelluk
Oct 14, 20162 min read
Insoluble Proteins – Inconsolable!
You may have had the experience! An expression project is going really well. You inserted your gene into a baculovirus vector...
14 views0 comments
yelluk
Sep 22, 20163 min read
Protein Expression and Directed Mutations
We get asked to do many service projects for customers where they want to produce a modified form of a protein for structural/functional...
10 views0 comments
yelluk
Aug 18, 20163 min read
Louis Pasteur and Insect Viruses
We told you last time about our trip to the Society for Invertebrate Pathology meeting in Tours, France. This was another highly...
3 views0 comments
yelluk
Jun 29, 20163 min read
Producing Virus-Like Particles (VLPs) in Insect Cells
Using baculovirus expression vectors to produce virus-like particles (VLPs) is not new. Intact, non-infectious poliovirus1 and...
47 views0 comments
yelluk
Jun 14, 20163 min read
Producing Secreted Proteins
We had promised to consider the production of virus like particles in this blog, but have postponed this topic to a later date after the...
20 views0 comments
yelluk
May 31, 20163 min read
Protein Purification
After scaling up recombinant baculovirus-insect cell cultures (blog) we now come to what can be a very tricky part of the operation,...
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yelluk
May 13, 20164 min read
Scaling Up Protein Production
Once you have decided on the optimal conditions for producing your recombinant protein target in baculovirus-infected insect cells, your...
17 views0 comments
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