yelluk
Introduction to the Baculovirus Expression System
Baculoviruses are in many respects an odd choice for use as an expression system to make recombinant proteins. In their natural...
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EXPERTS IN BACULOVIRUS PROTEIN EXPRESSION
BAC TO THE FUTURE
yelluk
Jun 11, 20192 min read
Baculovirus P10 Structures and Role in Insect Cells
A blog on baculovirus P10 structures and the role in insect cells might appear a slightly odd topic for a blog from a company based on...
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yelluk
Jul 9, 20182 min read
Baculovirus Transfer Vector Plasmid Compatibility
A frequent customer query concerns baculovirus transfer vector plasmid compatibility with either flashBACTM or other systems for making...
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yelluk
Sep 20, 20172 min read
Baculovirus Transfer Vectors Compatible with flashBAC™
A question we are asked from time to time is to identify baculovirus transfer vectors compatible with our flashBAC™ system. We obviously...
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yelluk
May 2, 20173 min read
baculoQUANT Titration of Recombinant Baculoviruses
There are many methods available for the titration of recombinant baculoviruses. One of the most convenient is the use of the...
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yelluk
Jan 26, 20172 min read
Transduction of Whole Porcine Kidneys with BacMams
One of the unexpected offshoots of the baculovirus expression system has been its development as a vehicle for the delivery of genes to...
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yelluk
Jul 22, 20162 min read
Bac to Our Roots!
Ever wondered where the baculovirus insect cell expression system came from? The clue is in the name – of course it came from viruses...
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yelluk
Jul 14, 20163 min read
Baculovirus Gene Mutations and Protein Expression
Have you ever wondered why the baculovirus we use for most protein expression purposes is called AcMNPV? This is short for Autographa...
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yelluk
Jun 29, 20163 min read
Producing Virus-Like Particles (VLPs) in Insect Cells
Using baculovirus expression vectors to produce virus-like particles (VLPs) is not new. Intact, non-infectious poliovirus1 and...
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yelluk
Apr 11, 20163 min read
Longevity of Infectious Budded Baculovirus Stocks
A question that comes up repeatedly is the long term stability of recombinant budded baculovirus stocks. This is a particular issue now...
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yelluk
Mar 31, 20163 min read
Rapid Virus Titration by QPCR
Knowing the infectious titre of a recombinant virus stock is really important prior to testing for protein production in insect cells. ...
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yelluk
Mar 11, 20164 min read
Titrating Stocks of Recombinant Baculoviruses Using the Plaque Assay Technique
We were going to devote this blog to a comprehensive account of how you might determine the infectious titre of your virus stock. ...
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yelluk
Feb 25, 20165 min read
Production of High Titre Virus Stocks
In our last blog post we talked about the process of transfecting insect cells with virus DNA and how you could recognise success or...
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yelluk
Feb 10, 20163 min read
Transfection of Insect Cells with Baculovirus DNA
Independent of the method you use to produce recombinant viruses, at some point you have to transfect insect cells with virus DNA to...
87 views0 comments
yelluk
Jan 13, 20162 min read
To heat treat or not to heat treat calf serum, that is the question?
Ok, so the Bard is probably spinning in his grave at our blatant plagiarism of the script from the “Scottish Play” (it is bad luck to use...
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yelluk
Nov 13, 20152 min read
Baculovirus Protein Expression Made Easy – flashBAC!
The flashBAC™ system is a revolutionary platform technology for the production of recombinant baculoviruses in insect cells. It comprises...
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yelluk
Sep 23, 20142 min read
Producing Your Recombinant Baculovirus
There are 2 main methods through which you can happily produce recombinant baculoviruses, firstly by using systems based on homologous...
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yelluk
Aug 4, 20142 min read
Baculovirus with Benefits: Expression System Advantages
As with all protein expression systems the baculovirus has its advantages and set-backs, the trade-off for high levels of expression,...
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yelluk
Jul 17, 20143 min read
Growing Pains: The Life Cycle of the Baculovirus
Thankfully baculoviruses don’t go through a rebellious teenage phase. They do however have a well-defined 2 step life cycle. Here we will...
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yelluk
Jul 15, 20141 min read
First Things First: What Exactly Is A Baculovirus?
A quick introduction into baculoviruses and baculovirus expression vectors, brought to you by OET. Short answer: An insect virus which...
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