Service Detail

Cost

Time-scale

Deliverables

Gene synthesis or PCR cloning into transfer plasmid

from £1050- Large/complex genes may incur an extra charge

3-4 weeks

Agreed transfer plasmid with gene ready to make virus and full report

Sub-cloning into transfer plasmid

from £700 - Complex strategies may ncur an extra charge

2-3 weeks

Agreed transfer plasmid with gene ready to make virus and full report

Production of recombinant virus (50 ml) and test expression in 2 cell lines

£1250 flashBAC

£1350 flashBAC GOLD

£1450 flashBAC ULTRA

2-3 weeks

50 ml P1 virus stock, titrated by QPCR

Western blot test expression report

Optimisation of expression (moi, time to harvest, pellets/culture medium) by Western blot

£650 per cell line

2 weeks

Western blot and optimisation report with recommended culture conditions to optimise expression

Optimisation as above plus 1-2 ml samples sent to customer for analysis

£700 per cell line

2 weeks

As above plus 1-2 ml samples to test in own laboratory

Protein production (amplification of P2 virus stock and infection of cells to produce recombinant protein)

£500 per 1 L

£2000 per 5 L

(pro rata other volumes)

2 weeks

Amplification and titration of required amount of P2 virus (100-200ml/L protein)

Production of cell pellet and/or culture medium containing recombinant protein to ship to customer or for use in 3.3

Protein production in monolayer T flasks

£300 per 500 ml virus

£375 per 40 T175 flasks

2 weeks

Amplification and titration of required amount of P2 virus (500 ml)

Production of cell pellet and/or culture medium containing recombinant protein to ship to customer or for use in 3.3

Protein purification using His tag (1 column)

£1250 (up to 5 L)

1 week

Purified protein and report including gel analysis

Protein purification using His-tag (1 column) plus cleavage

£1550 (up to 5 L)

1 week

Purified, cleaved protein including gel analysis

Additional column steps

£300 per column

1 week

Purified protein and report including gel analysis

Alternative purification e.g. strep/HA tag

Dependent upon protocol – please enquire

1 week

Purified protein and report including gel analysis

Characterisation of purified protein by Mass Spec

Depends on analysis – please enquire

2 weeks

Report on findings of Mass Spectrometric analysis

All-in-one basic gene expression service: gene synthesis/PCR cloning, making virus, test expression, 1 L protein production (1.1, 2.1, 3.1)

£2,800

6-8 weeks

Transfer plasmid as agreed with gene

50 ml P1 virus stock (FB GOLD)

Test expression western blot

1 L protein (as pellet/culture medium) and residual P2 virus stock (if any)

All-in-one basic gene expression service: sub-cloning then as above

£2,500

5-7 weeks

As above

All-in-one basic gene expression and protein purification service.  As above but including one-step His-tag protein purification

£3,800

7-9 weeks

Transfer plasmid as agreed with gene

50 ml P1 virus stock

Test expression western blot

Purified protein and residual P2 virus stock (if any)

All-in-one basic gene expression and protein purification service. As 4.1 but sub-cloning gene and one-step His tag protein purification

£3,500

6-8 weeks

As above

All-in-one basic gene expression service using clients own transfer plasmid to 1 L protein production

£1800

4-5 weeks

50 ml P1 virus (titrated by QPCR)

Test expression Western blot

1 L protein as pellets/culture medium and residual P2 virus (if any)

All-in-one basic gene expression service using clients own transfer plasmid to 1 L protein purification (2.1, 3.1, 3.2)

£2950

5-6 weeks

50 ml P1 virus (titrated by QPCR)

Test expression Western blot

Purified protein and residual P2 virus (if any)

Virus amplification in Sf9 cells and titration by QPCR

≤500 ml: £300

˃500ml: £200 per  500 ml + £80

1 week

Virus of known titre (by QPCR)

Virus amplification in Sf9 cells and titration by plaque-assay

≤500 ml: £400

˃500ml: £200 per 500 ml + £180

2 weeks

Virus of known titre (by plaque-assay)

Virus titration by plaque-assay to determine accurate titre

£180 per virus

1 week

Accurate titre of virus (essential for titration of older virus stocks)

Virus titration by QPCR

˂5: £80 per virus

≥5: £60 per virus

1 day

Accurate titre of fresh virus stocks

Recovery of old virus:

2-3 rounds of virus amplification as required, initial titration by plaque-assay and interim/final by QPCR.   Test expression optional.

£850 per virus with test

£700 per virus w/o test of expression

tbc

50 ml high titre virus stock

Western blot of test expression (if required)

Virus safe deposit:

Preparation of 40 1 ml vials virus (20 stored at OET; 20 at ECACC.   Test recovery of virus and gene expression.

£950 per virus

£210 annual OET charge

£700 annual test recovery and expression

ECACC storage charges are extra

tbc

50 ml high titre virus

 40 x 1 ml vials – test recovery and expression

20 vials stored at OET

20 vials stored ECACC

Annual recovery and test expression (as required)

Use of own cell line in expression work.  Initial recovery of cells and weekly passaging.  Use of cells in all experimental work

£355 initial charge

£210 annual storage (up to 20 vials

£120 weekly maintenance charge

Full data sheets for initial recovery and weekly passaging using agreed medium

Shipping charges

As charged to us

n/a

Added to all invoices for shipping virus, cell pellets, culture medium, purified protein – as agreed and at cost.

We also ship using customers own carrier if preferred or local customers can collect by agreement.