Oxford Expression Technologies

pOET1 and 2 are baculovirus transfer plasmids
designed for high level expression of foreign genes
under the powerful AcMNPV polyhedrin (polh)
promoter. They have a Col E1 origin of replication
and an ampicillin resistance gene for selection in
E. coli.
The vectors are smaller than other available
transfer vectors (4541 bp) which greatly facilitates
the cloning steps. The polh coding sequences
have been replaced by a multiple cloning site
containing 14 unique restriction sites for insertion
of the foreign gene in the correct orientation.
pOET2 has the same MCS as pOET1, but in the
reverse orientation.
The PacI site at the end of the MCS provides
translational stop codons in all three reading
frames for expression of truncated proteins. The
AcMNPV sequences flanking the MCS facilitate
recombination with the virus DNA to insert the
expression cassette into the polh locus. As well as
our own flashBAC platform these vectors can also
be used with any other baculovirus system that
uses homologous recombination to insert the
foreign gene into the virus genome.