Oxford Expression Technologies

pOET1 is a baculovirus transfer vector designed for high level expression of foreign genes under the powerful AcMNPV polyhedron polh promoter. The vector is smaller than other available transfer vectors (4541bp) which greatly facilitate the cloning steps. It has a bacterial origin of replication and an ampicillin resistance gene for selection in E. coli. The polh sequences have been replaced by a multiple cloning site containing 14 unique restriction sites for insertion of the foreign gene in the correct orientation. The AcMNPV sequences flanking the gene in the transfer vectors MCS allow recombination with the viral DNA to insert the expression cassette into the polh locus. pOET1 is compatible with any baculovirus system that utilizes homologous recombination in insect cells. including;

flashBAC (OET Ltd)
flashBACGOLD (OET Ltd)
flashBACULTRA (OET Ltd)
)BacMagic (Novagen)
BacMagic2 (Novagen)
BacVector 1000 (Novagen)
BacVector 2000 (Novagen)
BacVector 3000 (Novagen)
BacPAK6 (Clontech)
BaculoGold (Clontech)
BaculoBright (Clontech)
ProGreen (AbVector)
ProFold (AbVector)
ProEasy (AbVector)
Sapphire (Orbigen)