Oxford Expression Technologies

The Sf9 cell line has become the workhorse of the baculovirus expression system, being used worldwide for the production of recombinant proteins. OET is now pleased to offer this tried and tested cell line for sale to our customers The cells are sold in batches of 1x10⁷ cells. Please note this product ships on dry ice so additonal shipping charges may apply.

Description

Sf9 cells are a clonal isolate from Spodoptera frugiperda (Fall Armyworm) IPLB-Sf21-AE cells. The Sf9 cells are adapted to serum-free suspension culture in baculoGROW media. The cells can be used for transient or stable expression of recombinant proteins, as a monolayer for transfection and production of recombinant baculovirus or for the propogation of baculovirus stocks. The Sf9 cells can be cultured as monolayer cultures in T-flasks and or in suspension cultures in shake flasks. The cells are prepared from lowpassage cells (<30 passages in baculoGROW). Frozen cells can be thawed and used directly in suspension culture or as a monolayer culture.

 Shipping and Storage 

Cells are shipped on dry ice and are supplied in a cryogenic vial containing 1x10⁷ cells. Cells were frozen in a freezing medium composed of 50% fresh serum free medium, 50% conditioned serum free medium and Dimethyl Sulfoxide (DMSO) to a final concentration of 10%. Store cells in liquid nitrogen (vapour phase)