Oxford Expression Technologies

flashBAC baculovirus expression vector DNA kits

The simplest and the quickest baculovirus protein expression system! The flashBAC™ system is a new platform technology for the production of recombinant baculoviruses, genetically optimized for producing 100% recombinant virus in a single step by eliminating the need for plaque purification. The genetic optimizations not only prevent parental virus from replicating, but also significantly increases protein yield. There are a variety of flashBAC expression vectors, each varying in the number of genes deleted from the baculovirus genome to suit expression of different proteins. The original flashBAC vector is a great choice for projects where the gene to be expressed is likely to be simple and expressed in the cytoplasm or nucleus. flashBACGOLD is enhanced by the deletion of the chitinase and cathepsin protease genes. It provides superior levels of expression for any protein that is destined for secretion or to be inserted in the membrane, or for any protein that might be particularly liable to degradation. flashBAC ULTRA, the latest development of the flashBAC technology, contains deletions of the chitinase and cathepsin genes, as above, but also deletions of the p10, p74 and p26 genes and provides the best choice for the most difficult to express proteins. Suitable for cytoplasmic, nucleus, secreted and membrane proteins. The flashBAC selection box contains a combination of flashBAC, flashBACGOLD and flashBACULTRA in a single kit. If it is a starter kit you are after, baculoCOMPLETE is the perfect choice. Kit contains all the components needed to get started with recombinant protein expression in insect cells.